TOS FAQ MySpace FaceBook   [advanced search]
Register Today!
Stoner Forums - A Marijuana Friendly Community  
 
  Advertise your product or business on Stoner Forums
 
  
Register Today
Lost your password ?
 
 
 
Special Offers













 
Want the best marijuana experience?
Balance The Herb
Balance the Herb!

Cannabis Seeds

Nirvana Shop
Looking for fresh, viable seeds? Nirvana is the place to be! Order with us and you will be growing within days!
www.nirvana-shop.com
 

Get exposure from within the Counter Culture Community with Advertising on
StonerForums.com

Go Back   Stoner Forums - A Marijuana Friendly Community > The Stoner's GreenHouse > Advanced Techniques
Reload this Page Tissue Cultures and DNA Transformations...

Tissue Cultures and DNA Transformations...

Advanced Techniques

Reply
 
LinkBack Thread Tools Display Modes
Old 11-24-2008, 12:52 AM   #1
Loki_'s Avatar
Junior Stoner
My Mood:
 
Loki_ is trying to pack the bowl but will get the hang of it SOMEDAY


Cool Tissue Cultures and DNA Transformations...

Okay... hands up who's rockin the ganj on the cellular level?

Here's some pics of my gear in action...





-Loki
View Loki_'s Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Sponsored Links
Pass Your Next Drug Test
Old 11-24-2008, 12:59 AM   #2
MxMike's Avatar
CannaburnConnoisseur
My Mood:
 
MxMike is a glorious beacon of lightMxMike is a glorious beacon of light


Send a message via AIM to MxMike
Re: Tissue Cultures and DNA Transformations...

wtf is that man?!?!
That shit looks fucking cool!
__________________
origin View MxMike's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-24-2008, 01:26 AM   #3
Loki_'s Avatar
Junior Stoner
My Mood:
 
Loki_ is trying to pack the bowl but will get the hang of it SOMEDAY


Re: Tissue Cultures and DNA Transformations...

Quote:
Originally Posted by MxMike View Post
wtf is that man?!?!
That shit looks fucking cool!

Its a clean box for working with tissue cultures and DNA...

You can see some cultures in petri dishes (in the ziplock bags) and some shoots in test tubes on the top shelf...

Under the black shelf, next to the 'fridge' is a two-way hatch for moving things in and out. The 'fridge' is styrofoam and dry ice... The plastic containers hold all my mats and tools...

My goal is to extract the proper DNA fragments from marijuana and use them to transform the DNA from another plant to create a new THC containing plant.

-Loki
View Loki_'s Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-24-2008, 01:31 AM   #4
grimreefer's Avatar
Peace, Pot and Pride
My Mood:
 
grimreefer is just really nice


Send a message via MSN to grimreefer
Re: Tissue Cultures and DNA Transformations...

Quote:
Originally Posted by Loki_ View Post
Its a clean box for working with tissue cultures and DNA...

You can see some cultures in petri dishes (in the ziplock bags) and some shoots in test tubes on the top shelf...

Under the black shelf, next to the 'fridge' is a two-way hatch for moving things in and out. The 'fridge' is styrofoam and dry ice... The plastic containers hold all my mats and tools...

My goal is to extract the proper DNA fragments from marijuana and use them to transform the DNA from another plant to create a new THC containing plant.

-Loki
Wow, good luck with that, man.

If you need a guinnea pig let me know!
Haha
__________________
origin View grimreefer's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-24-2008, 01:39 AM   #5
MxMike's Avatar
CannaburnConnoisseur
My Mood:
 
MxMike is a glorious beacon of lightMxMike is a glorious beacon of light


Send a message via AIM to MxMike
Re: Tissue Cultures and DNA Transformations...

YOU ARE AN AMAZING GENIUS!!!!!!!!
OMFG I NEED TO DO THIS!
If only I had a way to help further this idea.
Keep on with that, I'll buy some when you get it right!
__________________
origin View MxMike's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Sponsored Links
HashBags
Old 11-24-2008, 03:49 AM   #6
Loki_'s Avatar
Junior Stoner
My Mood:
 
Loki_ is trying to pack the bowl but will get the hang of it SOMEDAY


Cool Re: Tissue Cultures and DNA Transformations...

Its a slow process... and I don't have sequencing equipment other than electrophoresis gels, which is just enough to tell me if my common flower DNA has changed after transforming it with DNA taken from my pot cultures. I'll have to grow everything out to see what effect the changed DNA has...

I'm thinking about making a video of the DNA extraction and transformation, but its such a pain getting into those gloves that I hate to take them on and off to run the camera...

-Loki
View Loki_'s Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-24-2008, 06:08 PM   #7
Osprey's Avatar
Solid.
My Mood:
 
Osprey is a name known to allOsprey is a name known to allOsprey is a name known to all


Send a message via Yahoo to Osprey
Re: Tissue Cultures and DNA Transformations...

Kudos man, best of luck on this project. You'll have a market if you succeed, that's for sure. I would just like to see first result when it grows!
__________________
Just beyond what you think you see, exist quantum possibility. Think about it.
origin View Osprey's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-24-2008, 10:15 PM   #8
MxMike's Avatar
CannaburnConnoisseur
My Mood:
 
MxMike is a glorious beacon of lightMxMike is a glorious beacon of light


Send a message via AIM to MxMike
Re: Tissue Cultures and DNA Transformations...

What plants are you planning on trying to infuse the Cannabis DNA with? You should use Hops man, that would be genius...

It's a sister plant to Cannabis and is a needed plant for the HUGE alcohol industry, therefore wouldn't be banned...

...and perhaps with hops being related to cannabis it may make the transformation more easily and produce THC.

Just an Idea...I really don't know what I'm talkin' about.
__________________
origin View MxMike's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-24-2008, 11:22 PM   #9
Osprey's Avatar
Solid.
My Mood:
 
Osprey is a name known to allOsprey is a name known to allOsprey is a name known to all


Send a message via Yahoo to Osprey
Re: Tissue Cultures and DNA Transformations...

There's that, plus you could pollinate commercial hops and they'd be screwed. No outlawing that. Who knows, you may wind up catastrophically fucking the brewing industry.
__________________
Just beyond what you think you see, exist quantum possibility. Think about it.
origin View Osprey's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-25-2008, 12:04 AM   #10
Loki_'s Avatar
Junior Stoner
My Mood:
 
Loki_ is trying to pack the bowl but will get the hang of it SOMEDAY


Cool Re: Tissue Cultures and DNA Transformations...

Quote:
Originally Posted by MxMike View Post
What plants are you planning on trying to infuse the Cannabis DNA with?
Right now, I'm using african violets. Mainly because they bloom readily and are covered with tricromes... just not THC containing tricromes... yet.

I've got a couple strains of praire grass that I'm going to try as well, but first things first... I'll see what the violets do and go from there..

-Loki
View Loki_'s Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Sponsored Links
Space Grinders
Old 11-25-2008, 12:06 AM   #11
Osprey's Avatar
Solid.
My Mood:
 
Osprey is a name known to allOsprey is a name known to allOsprey is a name known to all


Send a message via Yahoo to Osprey
Re: Tissue Cultures and DNA Transformations...

Ha! Prairie grass, perfect strain name.
__________________
Just beyond what you think you see, exist quantum possibility. Think about it.
origin View Osprey's Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 11-25-2008, 12:14 AM   #12
Loki_'s Avatar
Junior Stoner
My Mood:
 
Loki_ is trying to pack the bowl but will get the hang of it SOMEDAY


Re: Tissue Cultures and DNA Transformations...

Quote:
Originally Posted by Osprey View Post
Kudos man, best of luck on this project. You'll have a market if you succeed, that's for sure. I would just like to see first result when it grows!
Thanks. If it works, I'm just going to give it away... I'll try and find some growers to do a few grows of it and then have them give it to other growers.... I want to share, but I don't want to be teaching people to grow

-Loki
View Loki_'s Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Old 12-07-2008, 05:06 PM   #13
Loki_'s Avatar
Junior Stoner
My Mood:
 
Loki_ is trying to pack the bowl but will get the hang of it SOMEDAY


Cool Re: Tissue Cultures and DNA Transformations...

Cannabis TV :: Video :: tissue culture media prep

-Loki
View Loki_'s Photo Album  
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!
Reply With Quote
Sponsored Links
HashBags
Reply

Thread Tools
Display Modes



Bee Line Hawaii
Space Grinders
Advertise your product or business on Stoner Forums

 
 · Register
 · Calendar
 · Memberlist
 · Help / FAQ
 · Today's Posts
 
© 2006-2009 THC Media All Rights Reserved
Powered by vBulletin®
SEO by vBSEO 3.2.0
vBCredits v1.4 Copyright ©2007 - 2008, PixelFX Studios
Nirvana Seeds All times are GMT +1. The time now is 05:16 AM.

StonerForums.com is intended for persons age 18 years and older. Contents of this site are for informational purposes only. We accept no liability for user's actions.

StonerForums.com is hosted in the Netherlands.
Best viewed at 1024x768 resolution
Optimized for use with Firefox browser
 
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85